Human gut microbiota play an important role in the immune system and de novo synthesis of essential vitamins. Little is known, however, about how gut microbiota influence overall health and disease. Functional Profiling of the Microbiome will aid researchers, physicians, and patients in understanding the role of gut microbiota in human health and wellness. The proposed technology provides a way to quickly and easily identify the enzyme activity present in a sample. Coupled with existing metagenomics, this functional profile will provide insight into the effect of diet and medication use on the gut and the relationships between microbial functionality and disease progression.