GLASS PHASE PLATE FOR HIGH PRECISION WAVELENGTH EXTRACTION

Traditional localization microscopy uses photo-chemical blinking or switching of fluorescent molecular tags to generate super-resolution (SR) images. Obtaining multi-color images, however, requires filters, which limits the number of colors of fluorescence that can be used at one time.

The etched glass phase plate modifies the point-spread function to have a recognizable shape at each wavelength. Different spectral signals can then be measured without using filters or polarized spatial light modulators. The spectral information may also be rendered in conjunction with an SR image. The phase plate can be tailored to individual imaging needs by adding thin films to affect the slope of the dispersion curve. This may provide increased sensitivity to a desired range of wavelengths. The phase plate can be easily inserted into the Fourier plane of an existing microscope or imaging system.