Existing high-throughput, fluidics-based RNA sequencing systems are incompatible with short read length platforms and can only capture a single sequence. Additionally, variable regions of T-cell receptor pairs are separated during purification, and existing technology only allows capture of a single sequence making it difficult to accurately determine the existence and relative concentrations of receptor chains.
Bi-functional mRNA capture beads, synthesized using reversible oligonucleotide chain-blocking, isolate and amplify two different mRNA sequences while maintaining the pairing information for these sequences. The bead has a proprietary base that blocks chain elongation in order to capture and read the complete variable region of each chain. The multiple reads per bead will provide statistical conformation that the sequence is correct. Initial tests have demonstrated that two different capture sequences can be built onto a single bead, enabling specific capture and amplification of multiple different mRNA species.