SENSITIVE ASSAY FOR 5α-DIHYDROTESTOSTERONE

Circulating androgen 5a-dihydrotestosterone (DHT) is a major indicator of benign prostatic hyperplasia, which affects over three million men each year in the United States and can lead to prostate cancer. Additionally, polycystic ovarian syndrome (PCOS), which affects 8-10 percent of women, is caused by elevated androgen concentrations. PCOS causes conditions ranging from type 2 diabetes to obesity and heart disease. Immunoassays for testosterone and other androgens, however, are often inaccurate due to analytical interference and inaccurate results at low concentrations.

A simple, high-throughput assay uses specific derivitizing agents to improve detection of keto-steroids, such as DHT. This method has enhanced ionization efficiency and can detect analytes at low concentration levels.