TWO-ZONE FLOW-THROUGH PCR USING THIN FILMS

PCR is an inexpensive and robust technique for amplifying specific segments of DNA for gene analysis, DNA sequencing, DNA profiling, and diagnostic tests. The speed at which PCR can be performed depends on the time required to cycle through temperature dependent steps.
A novel PCR machine that combines a microfluidic card and simple thermal cycler performs PCR at extreme speeds by reducing cycling time to less than a second. Copper blocks surround the microfluidic consumable card to improve heat transfer and temperature control. This interaction reduces overall PCR time by enabling temperature equilibration in under 0.3 seconds. The device is more affordable than existing solutions and portable, facilitating use in point-of-care settings.